Articles
Bifidogenic Properties of Barley Seedling Extracts
Authors:
Yulia V. Zakharova, Tatyana V. Kotova, Olga M. Soboleva, Andrey S. Sukhikh, Violeta M. Le
Abstract:
The study subject is the bifidobacteria reproduction kinetics in the presence of barley seed extracts. A man run the research in order to study the influence of the extract nature of barley seedlings microgreens with different preparation variants for sowing on representatives of the genus Bifidobacterium in vitro. There were two strains used: Bifidobacterium bifidum 791 and Bifidobacterium bifidum 20. Dry seeds were exposed to microwave radiation with a power of 0.42 kW and 0.70 kW before sowing, the magnetron frequency was 2.45 GHz, the exposure was 11 sec. Then a man sprouted the grains for seven days. The researchers determined the sugar composition in the extracts by the Shimadzu LC-20 Prominence chromatograph. monitored the bacterial growth kinetics by the spectrophotometer SF-2000 (Experimental Design Bureau “Spectrum”, Russia), recording the optical density of the cuvette contents hourly for 20 hours. There was the reproduction stimulation of B. bifidum 791 and B. bifidum 20 in the exponential phase with an extract from barley seedlings treated with 0.42 kW microwave beams. Moreover, there were stable optical density indicators of B. bifidum 791 in the stationary phase, mainly when adding barley extract with maximum processing power. According to the chromatography data, the trietol content, a powerful antioxidant; and galactose, a bifidogenic prebiotic, increases in seed extracts with such preparation options. A man can use the barley extracts treated with microwave rays in biotechnological processes to reduce the adaptation time of bifidobacteria in periodic culture and to form a high population density in the stationary growth phase.
Keywords:
bifidobacteria; seedling extracts; sugar; growth kinetics
For citation:
Yulia V. Zakharova, Tatyana V. Kotova, Olga M. Soboleva, Andrey S. Sukhikh, Violeta M. Le. Bifidogenic Properties of Barley Seedling Extracts. Индустрия питания|Food Industry. 2022. Vol. 7, No. 1. Pp. 54–62. DOI: 10.29141/2500-1922-2022-7-1-7
