Articles
EPR spectroscopy in SOPs protocol using γ-radiation to ensure the dry mare’s milk technological quality
Authors:
Yu. V. Iliukhina, Roza T. Timakova, Ekaterina V. Pastushkova
Abstract:
The studies were conducted to establish the possibility of identifying samples of dry mare’s milk obtained by freeze-drying, pre-treated with different doses of γ-radiation, using the electron paramagnetic resonance (EPR) method, and the subsequent use of the established optimal doses of γ-radiation to achieve safety and organoleptic indicators in accordance with the requirements of TR CU 033/2013 “On the safety of milk and dairy products” and GOST R 52975-2008 “Canned milk. Dry mare’s milk. Technical conditions”. The SOPs protocol was followed at all stages of the overall research cycle: during treatment with different doses of γ-radiation from 0.5 to 6.0 kGy with a step of 0.5 kGy, subsequent validation according to the data of operational monitoring of the absorbed dose of γ-radiation using film dosimeters and quantitative identification based on the characteristic parameters of the EPR spectra (g-factor, amplitude, width, area), obtained using an instrumental approach on a spectrometer. With increasing radiation dose, an increase in signal intensity and its narrowing are observed. The appearance of multicomponent EPR spectra in the magnetic field range of 3260–3280 G with one main peak and two low narrow peaks (multiplet) is characteristic of radiation-treated dry mare’s milk containing more than 50% lactose. With an increase in the γ-radiation dose from 0.5 to 6.0 kGy, the absorbed dose increases by 12.03 times. Samples of dry mare’s milk treated with doses of 0.5–3.0 kGy fully complied with the requirements of GOST R 52975-2008. With an increase in the dose from 3.5 to 6.0 kGy, a change in color to light cream was noted, and a slight odor of pasteurization appeared. A correlation dependence of the QMAFAnM indicator on the applied dose of ionizing radiation was established. After treatment with a dose of 1.5 kGy, milk can be used for further industrial processing; when treated with doses over 2.0 kGy, it can be used for direct consumption without prior heat treatment.
Keywords:
electron paramagnetic resonance; protocol; γ-radiation; dry mare’s milk; dose; identification; validation
For citation:
Iliukhina Yu.V., Timakova R.T., Pastushkova E.V. EPR spectroscopy in SOPs protocol using γ-radiation to ensure the dry mare’s milk technological quality. Индустрия питания|Food Industry. 2026. Vol. 11, No. 1. Pp. 85–94. DOI: 10.29141/2500-1922-2026-11-1-9. EDN: SHLSFT.
