Evaluation of morphological parameters and primary chick embryo fibroblasts proliferative activity as a basis for obtaining growth factors in food biotechnology
Authors:
Alexander Yu. Prosekov, Darya V. Titarenko
Abstract:
Modern food biotechnology is actively developing cell-based technologies to create alternative sources of protein and biologically active substances. Chicken embryonic fibroblasts (CEFs) are a promising model for producing conditioned medium rich in growth factors. The purpose of the study is to obtain a primary culture of chicken embryonic fibroblasts (CEFs), evaluate their morphological properties, and determine the proliferative activity of the resulting culture in vitro. Chicken embryonic fibroblasts were isolated from 9-day-old chicken embryos using 0.25% trypsin-EDTA. The cells were cultured in DMEM/F12 medium with 10% fetal bovine serum at 37°C and 5% CO₂. The cell concentration during seeding was 4∙10⁶ cells/mL in each well. Viable cells were counted daily in a Goryaev chamber for 7 days. The isolated culture demonstrated a spindle-shaped morphology characteristic of fibroblasts. The growth curve had a sigmoid shape with a sequential change in the logarithmic phase (0–1 day), exponential phase (1–3 days), stationary phase (3–5 days), and declining phase (5–7 days). The maximum cell number (6.25∙10⁶/well) was reached on the third day. The authors found that primary CEFs possessed high proliferative potential, with the maximum cell concentration occurring on the third day of culture. The authors established that primary CEFs were characterized by high proliferative activity during the first few days (3–4 days) based on the data on the cell growth phase. It was found that depletion of the nutrient medium (glutamine and glucose) could have contributed to the slowdown in cell growth. The data obtained in this study provide a basis for the isolation and characterization of growth factors from conditioned medium for use in food biotechnology.
Keywords:
chicken embryo; morphological properties; embryonic fibroblasts (CEFs); primary culture; growth factor; proliferative activity
For citation:
Prosekov A.Yu., Titarenko D.V. Evaluation of morphological parameters and primary chick embryo fibroblasts proliferative activity as a basis for obtaining growth factors in food biotechnology. Индустрия питания|Food Industry. 2026. Vol. 11, No. 2. Pp. 83–90. DOI: 10.29141/2500-1922-2026-11-2-9. EDN: WSBIJO.
