Biotechnological aspects of obtaining collagen matrices from pork skin using enzymatic hydrolysis
Authors:
Anastasia Yu. Kolbina , Marina G. Kurbanova
Abstract:
The purpose of the study is to investigate the enzymatic hydrolysis of pork rind using the complex enzyme preparation "Protozyme 510" and to evaluate the feasibility of producing collagen matrices with specified structural and mechanical properties. The study examined the effects of enzyme concentration (0.2–1.0%), duration (6–48 h), and temperature (37–55 °C) on collagen yield, swelling coefficient, and film-forming capacity. Hydroxyproline content was determined spectrophotometrically and subsequently converted to collagen. The pH of the hydrolysate and aqueous extract of the washed samples was also assessed. Optimal hydrolysis conditions, ensuring the production of transparent films with the maximum collagen yield (78.5% of the initial content), were found to be achieved at an enzyme concentration of 0.5%, a temperature of 50 °C, and a hydrolysis time of 24 hours. The addition of 0.1% Tween-80 was shown to facilitate lipid removal and complete odor elimination, and slightly increased swelling (swelling coefficient of 0.44). The addition of CaCl2 does not significantly affect the hydrolysis parameters; however, its presence is necessary for the selective enzyme function. The authors confirmed experimentally that enzymatic hydrolysis of pork skin with the complex preparation "Protozyme 510" allows for the production of collagen matrices with high quality characteristics. The resulting films are characterized by a uniform structure, good elasticity, and high swelling capacity. The physicochemical parameters of the samples were determined: thickness 0.15–0.20 mm, moisture content 8–10%, density 1.2–1.3 g/cm3. The obtained results demonstrate the feasibility of applying the developed approach to the processing of secondary collagen-containing raw materials. The materials can be recommended for use in the food industry (production of edible casings, structure-forming agents, gelatin) and in medicine (wound dressings, hemostatic materials, tissue-engineered structures).
Keywords:
collagen matrix; pork skin; enzymatic hydrolysis; lipase; Protozyme 510; secondary raw materials
For citation:
Kolbina A.Yu., Kurbanova M.G. Biotechnological aspects of obtaining collagen matrices from pork skin using enzymatic hydrolysis. Индустрия питания|Food Industry. 2026. Vol. 11, No. 2. Pp. 66–74. DOI: 10.29141/2500-1922-2026-11-2-7. EDN: SUZYUA.
