Articles

Experimental Safety Proof of the Tonic Beverages Based on the Herbal Raw Materials

Authors:

Polyakov V.A., Poznyakovskiy V.M., Kotova T.V.

Abstract:

The scientific paper is devoted to the safety evidence experimental evaluation of the consumption of soft drinks based on the Rhodiola rosea extract by determining the activity of cytolysis biomarkers enzymes – alanine aminotransferase, aspartate aminotransferase, creatinekinase and lactate dehydrogenase. The study was performed on the reproductive male rats of Wistar line (n = 15, body weight – 461±23 g). They were watered daily using a pharyngeal pathfinder with drink containing 20 mg of the Rhodiola rosea extract in 100 cm3 of water in an amount of 4 µg of extract per 100 g of body weight. The infiltration was carried out three times a day for 21 days. 3 weeks after the beverage consumption the cytolysis biomarkers enzymes activity in plasma was determined. By the regression analysis the model for predicting changes in lactate dehydrogenase activity while consuming soft drinks with Rhodiola rosea extract is constructed. Models are characterized by close ties of effective and factor evidence with 95 % reliability level, the Fisher criteria adequate, and its significant coefficient in Student's t-criterion. The daily consumption of soft drinks with ginseng extract was determined not to cause significant changes in cytolysis bio-markers enzymes activity indicating its safety. This coincides with the assessment of forecast changes in the LDH activity. Developed mathematical forecasting modes has allowed to establish that changing the dosing regimen and concentration of the beverage with the Rhodiola rosea extract led to a change in LDH activity in the reference range.

Keywords:

soft drinks; Rhodiola rosea extract; biomarkers; cytolysi